MICROENCAPSULATION OF INDONESIAN POLYMER BIODIVERSITY IN WARTHON'S JELLY MESENCHYMAL STEM CELL (WJMSC)

نویسندگان

چکیده

Objective: WJMSC is a stem cell-derived from Wharton's Jelly the umbilical cord of pregnant women which has ability to differentiate into other cells that are osetogenic, myogenic, neurogenic, and hematopoietic. Stem cell microencapsulation coating technique expected act as delivery vehicle. This study aims make using various types natural polymers.
 Methods: (Wharton's Mesenchymal Cell) was cultured with supplemented Modified Eagle Medium (MEM) Alpha in incubator 37 °C 5% CO2. In this study, we investigated polymers (chitosan, glucomannan, inulin, fucoidan amylopectin) microencapsulation. Viability microencapsulate measured MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxy-phenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, condition medium (CM) determined measure EGF, IL-6, VEGF ELISA.
 Results: chitosan, oxidized amylopectin showed viability up 100%. The levels were increased all tested compared negative control.
 Conclusion: Tested fucoidan, not toxic successfully carried out.

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ژورنال

عنوان ژورنال: International Journal of Applied Pharmaceutics

سال: 2022

ISSN: ['0975-7058']

DOI: https://doi.org/10.22159/ijap.2022.v14s3.19